HPV16/18型原位杂交检测在子宫颈液基细胞学中的应用价值

江汉大学学报（自然科学版） ›› 2012, Vol. 40 ›› Issue (1): 106-108.

HPV16/18型原位杂交检测在子宫颈液基细胞学中的应用价值

黄萱¹，欧阳焱黎²，陈莹¹，陈琼霞¹，王成鑫³，刘丽江^1，3

1.江汉大学医学院，湖北武汉430056；
2.江汉大学第二附属医院，湖北武汉430050；
3.江大病理诊断所，湖北武汉430056

收稿日期:2011-08-29 出版日期:2012-02-20 发布日期:2013-11-07
通讯作者: 刘丽江（单位1955—)，女，教授，博士，研究方向:肿瘤病理学临床及基础。E－mail:liulijiang＠163.com
作者简介:黄萱单位1979—)，女，讲师，硕士，研究方向:临床肿瘤病理学。研究

Application Value of HPV 16/18 Detection by in Situ Hybridization in Liquid-based Thin-layer Cytology

HUANG Xuan¹,OUYANG Yan-li²,CHEN Ying¹,CHEN Qiong-xia¹,WANG Cheng-xin³,LIU Li-jiang^1,3

1. School of Medicine,Jianghan University,Wuhan 430056,Hubei,China;
2. The Second Affiliated Hospital,Jianghan University,Wuhan 430050,Hubei,China;
3. Jiangda Pathology Center,Jianghan University,Wuhan 430056,Hubei,China

Received:2011-08-29 Online:2012-02-20 Published:2013-11-07

摘要/Abstract

摘要： 目的评价薄层液基细胞学及HPV16／18型原位分子杂交检测在子宫颈病变中的应用价值。方法应用薄层液基细胞学技术及HE染色对112例患者行子宫颈脱落细胞学检查，按照TBS单位theBethesdareportsystem)细胞学分类标准分类无上皮内病变或恶性病变单位negativeforintroepitheliaormalignancy，NILM)，意义不明确的非典型鳞状上皮细胞单位atypicalsquamouscellofundeterminedsignificance，ASC－US)、低度鳞状上皮内病变单位Low－gradesquamousintraepitheliallesion，LSIL)、高度鳞状上皮内病变单位High－gradesquamousintraepitheliallesion，HSIL)，鳞状细胞癌单位squamouscellcarcinoma，SCC)。同时应用原位分子杂交方法对样本进行HPV16／18型检测。结果①在112例细胞涂片中，NILM16例单位14.3％);炎症病变26例单位23.2％);ASC－US44例单位39.3％);LSIL25例单位22.3％);HSIL1例单位0.89％);②112例细胞涂片中HPV16／18型阳性率随着子宫颈病变加重而增高，NILM及炎症、ASC－US、LSIL及HSIL各组间相比，NILM及炎症病变组与LSIL及HSIL组之间表达差异有统计学意义单位P＜0.05);③对5例细胞学HPV16／18型胞浆阳性病例的子宫颈活检标本进行HPV原位杂交对照检查，结果显示细胞核均呈阳性。结论子宫颈液基细胞学联合HPV16／18型原位分子杂交检测有助于发现HPV感染引起的子宫颈病变;HPV16／18型细胞涂片中胞浆阳性染色，可作为细胞学检查HPV阳性的判定指标。

关键词: 液基细胞学, HPV16／18型, 子宫颈细胞学

Abstract: ObjectiveTo evaluate the application value of liquid－based cytology and HPV 16／18 detection by in situ hybridization in cervical lesions. Methods112 cases were examined with liquid－based thin－layer cytology and stained with HE. According to the classification standard of TBS, the cervical lesions were divided into negative for intraepithelial or malignancy 单位 NILM); atypical squamous cell of undetermined significance单位 ASC－US); Low－grade squamous intraepithelial lesion 单位 LSIL); High－grade squamous intraepithelial lesion 单位 HSIL) and squamous cell carcinoma 单位 SCC). And HPV 16／18 of 112 cases were detected by in situ hybridization. Results① In the 112 cases, 16 cases 单位 14.3％) were NILM; 26 cases 单位 23.2％) were inflammatory; 44 cases 单位 39.3％) were ASC－US; 25 cases 单位 22.3％) were LSIL; 1 case 单位 0.89％) was HSIL. ② With the lesion aggravating, the positive rate of HPV 16／18 increased gradually. And there was a significant difference 单位 P＜0.05) between NILM, inflammation and LSIL, HSIL. ③ In cervical cytology, HPV 16／18 positive expression was located in cytoplasm in 5 cervical biopsy specimens. And it was also located in nucleus detected by in situ hybridization in the same specimens. ConclusionsLiquid－based cytology combining with HPV 16／18 detection by in situ hybridization is helpful for testing cervical lesion; cytoplasm HPV 16／18 positive staining may be used as a positive assessment marker in cervical cytology.

Key words: liquid－based cytology, HPV16／18, cervical cytology

中图分类号:

R737.330.4

黄萱，欧阳焱黎，陈莹，陈琼霞，王成鑫，刘丽江. HPV16/18型原位杂交检测在子宫颈液基细胞学中的应用价值[J]. 江汉大学学报（自然科学版）, 2012, 40(1): 106-108.

HUANG Xuan,OUYANG Yan-li,CHEN Ying,CHEN Qiong-xia,WANG Cheng-xin,LIU Li-jiang. Application Value of HPV 16/18 Detection by in Situ Hybridization in Liquid-based Thin-layer Cytology[J]. Journal of Jianghan University(Natural Science Edition), 2012, 40(1): 106-108.

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