关键词: 大蒜, 凝集素基因, 克隆, 生物信息学分析

Abstract: We obtained one novel garlic lectin gene with designed primers according to the sequence of Allium sativum agglutinin published in NCBI. The Chinese garlic bulb lectin gene，ASA-C，was cloned from RNAs by RT-PCR experiment. The entire length of the ASA-C was 546 bp containing a 471 bp ORF，which encoded 156 amino acids. The bioinformatics analysis of ASA-C was conducted by the website and analysis software. The results showed that the ASA-C was highly homology with the published garlic lectin ASAⅡ. It was speculated that there was a typical trans-membrane，one peptide biomarker，at the N-terminal domain. The sequence analysis showed three conserved motifs（Q-D-N-V-Y） for mannose-binding. The multiple sequence alignment and evolutionary analysis showed that the garlic lectin protein was highly conserved，which laid the fundation for the further development and utilization of the fundation of garlic lectin protein.

Key words: Allium sativum L., lectin gene, cloning, bioinformatics analysis