江汉大学学报(自然科学版) ›› 2021, Vol. 49 ›› Issue (4): 46-52.doi: 10.16389/j.cnki.cn42-1737/n.2021.04.006

• 生命科学 • 上一篇    下一篇

毛竹种子萌发及无菌水培技术研究

黄孝风a,b,钟秋蔚a,邹娜*a,b   

  1. 江西农业大学 a. 园林与艺术学院;b. 江西省竹子种质资源与利用重点实验室,江西 南昌 330045
  • 出版日期:2021-08-28 发布日期:2021-07-06
  • 通讯作者: 邹娜
  • 作者简介:黄孝风(1996— ),女,硕士生,研究方向:园林植物栽培与繁育。
  • 基金资助:
    国家自然科学基金青年科学基金项目(31300521);江西省自然科学青年基金重点项目(20171ACB21027);江西省研究生创新专项资金项目(YC2020-S261)

Research on Seed Germination and Aseptic Hydroponics of Phyllostachys edulis

HUANG Xiaofenga,b,ZHONG Qiuweia,ZOU Na*a,b   

  1. a. College of Landscape Architecture and Art;b. Jiangxi Province Key Laboratory of Bamboo Germplasm Resources and Utilization,Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China
  • Online:2021-08-28 Published:2021-07-06
  • Contact: ZOU Na

摘要: 采用室内可控的水培方法,研究了不同播前处理和播种方式对毛竹种子萌发以及不同水质和通气处理对毛竹幼苗水培的影响。结果表明:毛竹种子在40 ℃温水中浸泡过夜,去皮,75% 酒精浸泡5 ~ 10 s,10% NaClO 浸泡消毒20 min,去离子水冲洗6 ~ 8 遍后播入蛭石中生长状况最好,发芽率达61. 76%,且完全无污染;毛竹幼苗无菌水培以双滤水或无菌水为水质,在通氧加滤盘滤嘴的情况下污染率最低,但通氧与否对幼苗叶片数、苗高及地上地下生物量的影响差异不显著。

关键词: 毛竹, 播种, 水培体系, 发芽率, 污染率

Abstract: The effects of different pre-sowing treatments and sowing methods on the germination of Phyllostachys edulis seeds and the effects of different water quality and aeration treatments on seedlings' growth were studied by indoor controlled hydroponics methods. The results showed it was best that Phyllostachys edulis seeds were soaked in 40 ℃ water overnight, peeled, soaked with 75% alcohol for 5-10 s, soaked and disinfected with 10% NaClO for 20 minutes,washed with deionized water for 6-8 times and then planted into vermiculite,the germination rate could reach 61. 76% and there was no pollution during germination. The water culture of double-filtered water or sterile water was used as the water quality,the contamination rate of Phyllostachys edulis seedlings was the lowest under the condition of aeration and filter disk filters,but there were no significant effects of the oxygenation on leaf number, seedlings height and both aboveground and underground biomass of Phyllostachys edulis seedlings.

Key words: Phyllostachys edulis, seeding, hydroponics, germination rate, pollution rate

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