江汉大学学报(自然科学版) ›› 2020, Vol. 48 ›› Issue (6): 72-77.doi: 10.16389/j.cnki.cn42-1737/n.2020.06.010

• 生命科学 • 上一篇    下一篇

新品种紫辣椒和紫豇豆果皮花色苷含量测定

谢倩,阳晓婷,杨薇,李佳楠*   

  1. 江汉大学 生命科学学院生物技术系,湖北 武汉 430056
  • 出版日期:2020-12-28 发布日期:2020-12-18
  • 作者简介:谢倩(1995— ),女,硕士生,研究方向:生物制药。
  • 基金资助:
    LI Jianan

Determination of Anthocyanin Content in Peel of New Varieties of Purple Pepper and Purple Cowpea

XIE Qian,YANG Xiaoting,YANG Wei,LI Jianan*   

  1. Department of Biotechnology,School of Life Sciences,Jianghan University,Wuhan 430056,Hubei,China
  • Online:2020-12-28 Published:2020-12-18
  • Contact: 李佳楠

摘要: 辣椒和豇豆是人们餐桌中常见的两种蔬菜,从湖北省豆类(蔬菜)植物工程技术研究中心分别收集了3 种紫辣椒和10 种紫豇豆,采用有机溶剂提取法进行果皮花色苷的提取,利用pH 示差法对其花色苷的含量进行了测定。结果显示紫色蔬菜富含花色苷,在紫辣椒中,果皮花色苷含量最高的是江大紫辣椒2 号,为25. 936 3 mg/100g,花色苷含量的平均值为19. 011 5 mg/100g,花色苷含量超过平均值的有紫辣椒11-2-1、江大紫辣椒2 号;紫豇豆中果皮花色苷含量最高的是JD0567,为131. 203 6 mg/100g,花色苷含量的平均值为87. 355 mg/100g,其中JD0138、JD0821、JD0567、JD0140 的花色苷含量均超过平均值。本研究结果为今后选育出高花色苷含量的优良品种提供实验依据,为紫色辣椒和紫色豇豆中花色苷这一功能性成分的开发利用提供科学依据,有助于推广种植紫色蔬菜。

关键词: 新品种, 紫辣椒皮, 紫豇豆皮, 花色苷, pH 示差法

Abstract: Peppers and cowpeas are two common vegetables on people′s dinner tables. In this paper,3 kinds of purple peppers and 10 kinds of purple cowpeas were collected from Hubei Province Engineering Research Center for Legume Plant. The organic solvent extraction method was used to extract anthocyanins from the peel,and the anthocyanin content was determined by the pH differential method. The result showed that purple vegetables were rich in anthocyanins. Among the purple peppers , the highest anthocyanin content was JHUN purple pepper no. 2(25. 936 3 mg/100g),and the average anthocyanin content was 19. 011 5 mg/100g. Those whose anthocyanin content exceeded the average value were purple pepper no. 11-2-1 and JHUN purple pepper no.2.The highest anthocyanin content in purple cowpeas was JD0567(131. 203 6 mg/100g),and the average anthocyanin content was 87. 355 mg/100g. Among the purple cowpeas,the anthocyanin content of JD0138,JD0821,JD0567 and JD0140 all exceeded the average value. The results of this study provide an experimental basis for the breeding of excellent varieties with high anthocyanin content in the future,and the scientific basis for the development and utilization of anthocyanins in purple peppers and purple cowpeas as a functional component,which is also conducive to the promotion and cultivation of purple vegetables.

Key words: new varieties, purple pepper skin, purple cowpea skin, anthocyanin, pH differential method

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