江汉大学学报(自然科学版) ›› 2020, Vol. 48 ›› Issue (6): 48-55.doi: 10.16389/j.cnki.cn42-1737/n.2020.06.007

• 生命科学 • 上一篇    下一篇

响应面法优化微波提取牡丹果壳水溶性膳食纤维工艺

陈法志1,翟敬华1,刘克华2,李秀丽1,陈镇1,任钟毓1   

  1. 1. 武汉市农业科学院林业果树研究所,湖北 武汉 430075;2. 潜江市园林局,湖北 潜江 433100
  • 出版日期:2020-12-28 发布日期:2020-12-18
  • 作者简介:陈法志(1971— ),男,正高职高级工程师,硕士,研究方向:经济林育种与栽培。

Optimization of Microwave-Assisted Extraction of Water-Soluble Dietary Fiber from Peony Husk by Response Surface Methodology

CHEN Fazhi1,ZHAI Jinghua1,LIU Kehua2,LI Xiuli1,CHEN Zhen1,REN Zhongyu1   

  1. 1. Institute of Forestry and Fruit Tree,Wuhan Academy of Agricultural Sciences,Wuhan 430075,Hubei,China;2. Qianjiang Bureau of Parks and Woods,Qianjiang 433100,Hubei,China
  • Online:2020-12-28 Published:2020-12-18

摘要: 采用微波辅助法提取牡丹果壳中的水溶性膳食纤维(SDF)。分别选取柠檬酸质量分数、料液比、微波功率、微波时间进行单因素试验,在单因素试验的基础上,对提取的工艺条件进行响应面优化,选取对SDF 提取率影响大的料液比(A)、微波功率(B)、微波时间(C)3 个因素为因变量,以SDF提取率为响应值(Y),采用Design-Expert8.6.0 软件设计3 因素3 水平,进行响应面法试验,建立各因变量与响应值之间的回归方程,回归方程拟合良好,对SDF 的提取率影响大小为料液比>微波功率>微波时间。微波辅助提取SDF 的最佳工艺条件:料液比为1∶26. 19、微波功率为中火、微波时间为63. 6 s,SDF 提取率可达到45. 99%。建立了牡丹果壳SDF 微波提取工艺,对牡丹果壳资源深度开发利用具有指导意义。

关键词: 牡丹果壳, 水溶性膳食纤维, 微波辅助法, 响应面法

Abstract: To develop the resource utilization of peony husk, microwave-assisted extraction of water-soluble dietary fiber(SDF) from peony husk was used in this study. Single-factor experiments were performed by selecting the citric acid concentration, material/liquid ratio,microwave power,and microwave time. Based on the single-factor experiment, the response surface methodology was utilized to optimize the extraction process. The three variables of material/liquid ratio (A),microwave power (B),and microwave time(C)were selected as the dependent variables that had a large effect on the SDF extraction rate,and the SDF extraction rate was taken as the response value (Y). Design-Expert8.6.0 was used to design three factors and three levels,and a response surface test was performed to establish the regression equation between each dependent variable and response value. The regression equation fitted well,and the effect on the SDF yield was material/liquid ratio > microwave power > microwave time. The optimal process conditions for microwave-assisted extraction of SDF were as follows:material/liquid ratio was 1∶26. 19,microwave power was medium fire,and the microwave time was 63. 6 s. Under such conditions,the SDF extraction rate could reach 45. 99%. In this study,the SDF microwave extraction technology of peony husk was established, which had a guiding significance for the in-depth development and utilization of peony husk resources.

Key words: peony husk, water-soluble dietary fiber, microwave-assisted extraction, response surface analysis

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