关键词: 豇豆花苞, 内生菌, 不动杆菌, 革兰染色, 序列分析

Abstract: The entophyte bacteria（wjhb）was isolated from the cowpea flowers in cowpea planting base in Wuhan，which was the dominant strain in the cowpea florescence. The proper temperature range for the entophyte bacteria culture was 16～43 ℃ and the Gram staining result was negative. The biochemical experiment results showed the entophyte bacteria belonged to Acinetobacter sp. and the biochemical character was up to the Acinetobacter sp.. The 16SrDNA sequence PCR results showed the similarity of 16SrDNA sequence with Acinetobacter sp. reached 98%. The entophyte Acinetobacter sp.（wjhb）in this paper is an ideal candidate of the host for cowpea engineered bio-control bacteria.

Key words: cowpea, endophytic bacteria, Acinetobacter sp., Gram staining, sequence analysis