关键词: 唾液酸化N-聚糖, 氧乙酰化, 同分异构体, 双衍生, UPLC-MS/MS

Abstract: The sialylated N-glycans from freshwater crucian carp serum are rich in O-acetylation modification. This article provided an in- depth analysis of the O- acetylation distribution in dual modifications sialylated N-glycans released from the crucian carp serum with UPLC-Orbitrap-MS/MS. Uneven distribution of O- acetylation was identified in sialylated N- glycans isomers. The results enriched the biological information of glycomics of crucian carp. The established method has a wide application prospect in the research of glycan modification and the search for glycoprotein biomarkers.

Key words: sialylated N-glycans, O-acetylation, isomers, dual modifications, UPLC-MS/MS