胆酸钠多克隆抗体的制备及其快速检测方法研究

江汉大学学报（自然科学版） ›› 2012, Vol. 40 ›› Issue (2): 77-81.

胆酸钠多克隆抗体的制备及其快速检测方法研究

陈江源¹，刘洁²，蔡建中¹，付云洁³，刘志国³

1.江汉大学卫生技术学院，湖北武汉430016；
2.江汉大学文理学院，湖北武汉430056；
3.武汉工业学院生物与制药工程学院，湖北武汉430023

出版日期:2012-04-20 发布日期:2013-11-07

The Study on the Preparation of Sodium Cholate Polyclonal Antibody and its Fast Determination Method

CHEN Jiang－yuan¹,LIU Jie²,CAI Jian－zhong¹,FU Yun－jie³,LIU Zhi－guo³

1. School of Health Science,Jianghan University,Wuhan,430016,Hubei,China;
2. College of Arts ＆ Science,Jianghan University,Wuhan,430056,Hubei,China;
3. School of Biotechnic and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,Hubei,China

Online:2012-04-20 Published:2013-11-07

摘要/Abstract

摘要： 目的探索用胶体金免疫层析法快速检测胆酸钠含量。方法用碳二亚胺法合成的免疫抗原胆酸钠－BSA，免疫新西兰白兔，制备抗胆酸钠多克隆抗体。用酶联免疫吸附法(ELISA)鉴定特异性抗体效价。制备胆酸钠—OVA抗原—胶体金复合物，组装检测试纸。结果胆酸钠抗体的效价为1∶80000，建立的胶体金快速测定法对胆酸钠的检测限为12μg／mL。结论试纸条法是快速检测胆汁酸含量的有效方式，在临床检测方面具有广阔的运用前景。

关键词: 胆酸钠, 多克隆抗体, ELISA, 胶体金, 试纸条

Abstract: Objective:To develop colloidal gold immunochromatographic(IC) method for rapidly detecting the concentration of Sodium Cholate. MethodsSodium Cholate－BSA immunogen was obtained via carbodiimide method. Then the immunoantigen was injected into New Zealand rabbits to prepare the anti－sodium cholate polyclona antibody according to routine immunity procedure. The specific antibody of Bile acid natrium was identified by indirect enzyme linked immunosorbent assay (ELISA). ResultsThe sodium cholate－OVA antigen－colloidal gold compoundswere made for test strip preparation. It was proved that the specificity of the antibody was1 ∶ 80 000, and the detectability was 12 μg／mL by the method. ConclusionAccordingly, IC test strip method is effective and sensitive for rapidly detecting sodium cholate, which will probably have a broad clinical application prospect.

Key words: sodium cholate, polyclonal antibody, ELISA, colloidal gold, test strip

中图分类号:

R392.7

陈江源，刘洁，蔡建中，付云洁，刘志国. 胆酸钠多克隆抗体的制备及其快速检测方法研究[J]. 江汉大学学报（自然科学版）, 2012, 40(2): 77-81.

CHEN Jiang－yuan,LIU Jie,CAI Jian－zhong,FU Yun－jie,LIU Zhi－guo. The Study on the Preparation of Sodium Cholate Polyclonal Antibody and its Fast Determination Method[J]. Journal of Jianghan University(Natural Science Edition), 2012, 40(2): 77-81.

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