关键词: 体细胞, 克隆西农莎能奶山羊, 微卫星DNA 分析, 电泳, 银染

Abstract: Objective To identify two Xinong Saanen dairy goats whom were obtained through somatic cell cloning technology with microsatellite DNA technology. Methods Extracting genomic DNA of two cloned Xinong Saanen dairy goats，two recipient Xinong Saanen dairy goats，donor Xinong Saanen dairy goat and two control group Xinong Saanen goats，then microsatellite DNA analysis was performed with microsatellite DNA sequences which were amplified from five pairs significant different polymorphic primer. Results Genotype of two Xinong Saanen dairy goats are the same as the donor cells，but the microsatellite DNA polymorphism of recipient goat and control group goat are different from the above mentioned. Conclusion Genomic DNA of two Xinong Saanen dairy goats come from the donor cells.