关键词: 橙皮素, 水浸束缚应激, 空肠损伤, 抗氧化作用, 炎症反应

Abstract: Objective To investigate the protective effects of hesperetin on jejunal injury in mice subjected to water immersion restraint stress（WIRS）. Methods A WIRS model was established，with mice randomly assigned to four groups：control，model，low-dose hesperetin（50 mg/kg），and high-dose hesperetin（100 mg/kg）. Mice in the hesperetin groups were administered their respective doses for 7 consecutive days before undergoing the stress protocol. The protective effects of hesperetin were assessed by measuring serum levels of AST and ALT，tissue MDA content，and T-SOD activity，as well as serum TNF-α and IL-1β levels via ELISA. Histopathological analysis of the jejunum tissue was performed using HE staining，and villus height，crypt depth，and the villus height to crypt depth ratio were quantified. Results HE staining revealed that both low- and high-dose hesperetin groups significantly reduced serum AST and ALT levels，decreased MDA content，enhanced T-SOD activity，and suppressed TNF-α and IL-1β expression compared to the model group. Furthermore，hesperetin treatment resulted in increased villus height， reduced crypt depth，and an improved villus height to crypt depth ratio. HE staining indicated that hesperetin significantly alleviated jejunal tissue injury in stressed mice. Conclusion Hesperetin exerts significant protective effects against jejunal injury induced by WIRS in mice. This protective action is likely mediated by its antioxidant properties， antiinflammatory effects，and ability to promote intestinal tissue repair.

Key words: hesperetin, water immersion restraint stress, jejunal injury, antioxidant effect, inflammatory response