江汉大学学报(自然科学版) ›› 2025, Vol. 53 ›› Issue (5): 16-22.doi: 10.16389/j.cnki.cn42-1737/n.2025.05.002

• 汉江流域特色生物资源保护与利用 • 上一篇    

油菜EMS诱变群体萌发期耐盐突变体筛选与鉴定

何 棱1,曾长立1,南建军2,曹永胜2,沈金雄2,3,傅廷栋3,万何平*1   

  1. 1. 江汉大学 生命科学学院,湖北省汉江流域特色生物资源保护开发与利用工程技术研究中心, 湖北 武汉 430056;2. 湖北国科高新技术有限公司,湖北 武汉 430205; 3. 华中农业大学 作物遗传改良全国重点实验室,湖北 武汉 430070
  • 发布日期:2025-10-22
  • 通讯作者: 万何平
  • 作者简介:何 棱(2001—),女,硕士生,研究方向:油菜逆境生物学。
  • 基金资助:
    国家生物育种重大项目(2022ZD04010);国家自然科学基金项目(U22A20469)

Screening and Identification of Salt-tolerant Mutants in the Germination Stage of Rapeseed EMS Mutagenesis Population

HE Ling1,ZENG Changli1,NAN Jianjun2,CAO Yongsheng2,SHEN Jinxiong2,3, FU Tingdong3,WAN Heping*1   

  1. 1. School of Life Sciences,Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin,Jianghan University,Wuhan 430056,Hubei,China;2. Hubei National Science Hi-Tech Co. ,Ltd. ,Wuhan 430205,Hubei,China;3. National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,Hubei,China
  • Published:2025-10-22
  • Contact: WAN Heping

摘要: 以‘中双11号’油菜品种经甲基磺酸乙酯(ethylmethanesulfonate,EMS)诱变获得的276 份稳定的M4株系为材料,在种子萌发期进行精准耐盐性鉴定,成功筛选出29份耐盐性显著增强 的突变体。其中,6份突变体材料的种子萌发期耐盐性达到耐盐品种‘华油杂62’的水平,2份突 变体的耐盐性表现优于‘华油杂62’。同时还优化了一个基于EMS诱变改良油菜耐盐性的技术 流程。本研究首次探究了应用EMS诱变技术提升油菜耐盐性的可能性,为耐盐碱油菜新品种的 快速选育提供了宝贵的种质资源和技术路径。

关键词: 甘蓝型油菜, 萌发期, 耐盐性, 化学诱变, EMS诱变剂

Abstract: 276 stable M4 lines derived from the rapeseed cultivar ′Zhongshuang 11′ via ethyl methanesulfonate(EMS)mutagenesis were subjected to precise salt-tolerance screening at the germination stage,which identified 29 mutant lines with significantly enhanced salt- tolerance. Among these,6 mutants exhibited salt-tolerance comparable to the salt-tolerant cultivar ′ Huayouza 62′ at the germination stage,while 2 mutants outperformed ′Huayouza 62′ in salt-tolerance. Additionally,this study optimized a technical workflow for improving rapeseed salt-tolerance through EMS mutagenesis. This work marks the first exploration of EMS mutagenesis for enhancing salt-tolerance in rapeseed,providing valuable germplasm resources and a technical pathway for rapid breeding of saline-alkali-tolerant rapeseed varieties.

Key words: Brassica napus L., germination stage, salt-tolerance, chemical mutagenesis, EMS mutagen

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