关键词: 甘蓝型油菜, 氮利用效率, 表型性状, 生理生化指标

Abstract: Based on the nitrogen concentration environmental gradient for Brassica napus （B. napus）growth established through our research group′s exploration，we investigated the differences in phenotypic and physiological-biochemical indices between the high nitrogen use efficiency（NUE）B. napus material 10-Chong 32 and the low-NUE material WH- 17 under three nitrogen concentrations：low nitrogen （0. 3 mmol/L），normal nitrogen （6. 0 mmol/L），and high nitrogen（15. 0 mmol/L）. The results showed that under all growth conditions，the dry weight and fresh weight values of 10-Chong 32 were consistently higher than those of WH-17. The root activity-related parameters of 10-Chong 32 demonstrated superior performance compared to WH-17. Additionally，10-Chong 32 exhibited better photosynthesis-related metrics than WH-17， with significant differences observed in nitrogen metabolism-related enzyme activity levels between the two materials. The analysis reveals that B. napus materials with different nitrogen utilization efficiencies display distinct phenotypic and physiological-biochemical characteristics under varying nitrogen concentrations， thereby laying a theoretical foundation for subsequent exploration of the physiological- biochemical and molecular mechanisms underlying NUE in B. napus，as well as for breeding high-NUE B. napus varieties.

Key words: Brassica napus, nitrogen use efficiency （NUE）, phenotypic traits, physiological-biochemical indices