江汉大学学报(自然科学版) ›› 2025, Vol. 53 ›› Issue (3): 37-47.doi: 10.16389/j.cnki.cn42-1737/n.2025.03.005

• 生命科学 • 上一篇    

基于GWAS的超甜玉米种子发芽率调控基因预测

崔妮妮,魏贺勤,余育林,余海兵,程昕昕**   

  1. 安徽科技学院 农学院,安徽 凤阳 233100
  • 发布日期:2025-06-30
  • 通讯作者: 程昕昕
  • 作者简介:崔妮妮(1999—),女,硕士生,研究方向:甜玉米遗传育种。
  • 基金资助:
    国家自然科学基金委员会应急管理项目(31440066);安徽省高校自然科学研究重大项目 (KJ2019ZD57);安徽科技学院科研发展基金项目(FZ230126)

Gene Prediction for Regulating Germination Rate of Super Sweet Corn Seeds Based on GWAS

CUI Nini,WEI Heqin,YU Yulin,YU Haibing,CHENG Xinxin   

  1. College of Agriculture,Anhui Science and Technology University,Fengyang 233100,Anhui,China
  • Published:2025-06-30
  • Contact: CHENG Xinxin

摘要: 为探索调控超甜玉米种子发芽率的显著关联位点及候选基因,对100份超甜玉米自交系 种子发芽率相关性状进行测定,并利用超甜玉米56K基因芯片,采用MLM(Q+K)模型,通过 GWAS分析,共关联定位到5个与超甜玉米种子发芽率有显著关联的SNP位点,并在上下游 200 kb 的置信区间内,发现了43个已有功能注释基因,基于文献比较得出Zm00001eb057130、 Zm00001eb057170、Zm00001eb057030、Zm00001eb145440、Zm00001eb145480、Zm00001eb057150、 Zm00001eb145520、Zm00001eb057110这 8个基因是调控超甜玉米种子发芽率的候选基因,其中有 5 个候选基因在种子的胚胎发育中发挥作用,从而直接影响种子的发芽率,有3个候选基因主要 通过参与种子的胚根生长发育间接影响种子的发芽率。本研究结果可为选育高发芽率的超甜玉 米品种提供理论支撑,为精细定位和克隆发芽率相关性状基因提供参考。

关键词: 超甜玉米, 发芽率, 全基因组关联分析, 候选基因

Abstract: The germination rate-related characters of 100 inbred lines of super sweet corn were measured to explore the significant associated loci and candidate genes regulating the germination rate of super sweet corn seeds. Using the super sweet corn 56K gene chip,the MLM(Q + K)model was adopted to locate a total of 5 SNPs correlated with the germination rate of super sweet corn seeds through GWAS analysis,and 43 functionally annotated genes were found within the upper and lower 200 kb confidence interval. Based on literature comparison, 8 candidate genes (Zm00001eb057130, Zm00001eb057170, Zm00001eb057030,Zm00001eb145440,Zm00001eb145480,Zm00001eb057150,Zm0000 1eb145520, Zm00001eb057110) were found to be related to the regulation of seed germination rate of super sweet corn. Among them,5 candidate genes played a role in the embryonic development of seeds,thus directly affecting the germination rate of seeds,and 3 candidate genes indirectly affected the germination rate of seeds,mainly by participating in the root growth and development of seeds. The results of this study can provide theoretical support for breeding super sweet corn varieties with high germination rates,which provides a reference for fine mapping and cloning of genes related to germination rate.

Key words: super sweet corn, germination rate, genome-wide association analysis, candidate genes

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