关键词: 分子标记, 遗传指纹图谱, 玉米纯度, 瑞华玉288

Abstract: To construct the fingerprint of the new hybrid corn variety Ruihuayu 288，the InDel primers suitable for its purity identification were screened. The fingerprint was constructed with InDel molecular marker technology，the purity of Ruihuayu 288 seeds was identified with the primers，and the identification results were verified by combining the field community planting identification method. The results showed that 20 pairs of primers with good polymorphism，clear，and stable primers were screened out of 52 pairs of InDel primers to construct the fingerprint of Ruihuayu 288，and the polymorphism was 38. 46%. Eleven pairs of primers in 20 pairs of primers could be used for the purity identification of maize hybrids Ruihuayu 288，and the purity of Ruihuayu 288 identified with 3 pairs of primers was 96. 26%，which was highly consistent with the results of 98. 13% of the field community planting identification. The results indicate that the molecular identification method can quickly identify the purity of maize hybrids， which is conducive to the monitoring of maize seed quality.

Key words: molecular markers, genetic fingerprint, corn purity, Ruihuayu 288