关键词: ：3，5-二叔丁基水杨酸锌, 纳米粒, 三阴性乳腺癌, 纳米沉淀法

Abstract: Objective Zinc 3，5-di-tert-butylsalicylate［Zn（DTBS）₂ ］has extremely low solubility in water and is prone to aggregation and precipitation，which reduces its efficacy. To overcome this defect，zinc 3，5-di-tert-butylsalicylate nanoparticles（ZnNPs）were prepared to improve the bioavailability of the raw drug and investigate its inhibitory effect on the activity of triple-negative breast cancer cells. Methods After ZnNPs were prepared by nanoprecipitation，the morphology，particle size，zeta potential，encapsulation efficiency， and drug loading of the nanoparticles were determined，and its inhibitory effect on human triple-negative breast cancer cells MDA-MB-231 was detected by MTS assay. Results ZnNPs had a solid spherical structure of uniform size，with diameter of（240 ± 18）nm，ζ potential of（-35 ± 8）mV，encapsulation efficiency of（82. 66 ± 1. 98）% ，and drug loading of（11. 77 ± 0. 28）%. ZnNPs inhibited the activity of triple-negative breast cancer cells in a concentration-and time-dependent manner from the concentration of 50 μmol/L， and compared with the same concentration of free ［Zn（DTBS）₂ ］，ZnNPs showed stronger anticancer activity. ConclusionZnNPs can enhance the inhibitory effect of Zn （DTBS）₂ on triple-negative breast cancer cell activity.

Key words: zinc 3，5-ditert-butylsalicylate, nanoparticles, triple-negative breast cancer （TNBC）, nanoprecipitation